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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,201)
Mouse Secondary Antibodies (14,525)
Rabbit Secondary Antibodies (6,310)
Donkey Secondary Antibodies (4,319)
Rat Secondary Antibodies (1,237)
Sheep Secondary Antibodies (701)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Pig Secondary Antibodies (77)
Horse Secondary Antibodies (74)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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4,831 – 4,845 of 72,047 results
4,831

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 488 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 488
Applications Flow Cytometry,Immunomicroscopy
Form Purified
Isotype IgG
Concentration LYOPH
Antigen IgG (H+L)
Dilution Flow Cytometry 1:20-1:2000, Immunomicroscopy 1:20-1:2000
Formulation 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Classification Polyclonal
Immunogen Purified Sheep IgG (H&L)
Reconstitution Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on sheep IgG and light chains on all sheep immunoglobulins
4,832

Donkey anti-Goat IgG (H+L) Secondary Antibody, DyLight 755, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Goat
Host Species Donkey
Conjugate DyLight 755
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against goat IgG.
Primary or Secondary Secondary
4,833

Donkey anti-Rat IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 3 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Rat
Host Species Donkey
Conjugate Unconjugated
Applications ELISA,Immunoassay,Immunodiffusion,Immunohistochemistry,Immunoprecipitation,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 4.5mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Immunoassay
Classification Polyclonal
Immunogen Purified Rat IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep IgG.
4,834

Donkey anti-Mouse IgG (H+L) Secondary Antibody, DyLight 350, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Donkey
Conjugate DyLight 350
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Primary or Secondary Secondary
4,836

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Mouse
Host Species Donkey
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:2000 - 1:20000, ELISA 1:2000 - 1:20000, Immunohistochemistry 1:500 - 1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep IgG.
4,837

Invitrogen™ F(ab')2-Donkey anti-Sheep IgG (H+L) Secondary Antibody

F(ab')2-Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Sheep
Host Species Donkey
Conjugate Unconjugated
Applications ELISA
Form Liquid
Isotype IgG
Concentration >4.5 mg/mL
Antigen Sheep IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Chromatography
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.05% sodium azide; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
4,838

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 550, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 550
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Rabbit IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins
4,839

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Sheep
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.50mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Sheep IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on sheep IgG and light chains on all sheep immunoglobulins
4,840

Donkey anti-Human IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Antigen IgG (H+L)
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Purification Method Affinity Purified
Conjugate Unconjugated
Target Species Human
Host Species Donkey
Applications ELISA,Immunohistochemistry,Western Blot
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free with 0.01% Sodium Azide
Classification Polyclonal
Immunogen Human IgG whole molecule
Isotype IgG
Primary or Secondary Secondary
4,841

Pig anti-Human IgA/IgG/IgM (H+L) Secondary Antibody, Novus Biologicals™
SDP

Pig Polyclonal Antibody

Antigen IgA/IgG/IgM (H+L)
Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purification Method Ion exchange chromatography
Conjugate Unconjugated
Target Species Human
Host Species Pig
Applications Immunoelectrophoresis
Form Lyophilized
Formulation Lyophilized from PBS (pH 7.2) with No Preservative
Classification Polyclonal
Immunogen Highly purified IgG and pools of homogenous IgA and IgM isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Primary or Secondary Secondary
4,842

Pig anti-Human Kappa Light Chain Secondary Antibody, Novus Biologicals™
SDP

Pig Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Conjugate Unconjugated
Target Species Human
Host Species Pig
Applications Dot Blot,ELISA,Immunoblot,Immunocytochemistry,Immunofluorescence,Western Blot
Form Lyophilized
Antigen Kappa Light Chain
Purification Method Ion exchange chromatography
Gene Alias HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Formulation Lyophilized from PBS (pH 7.2) with No Preservative
Gene ID (Entrez) 3514
Classification Polyclonal
Immunogen A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
Primary or Secondary Secondary
4,843

Pig anti-Human IgE Fc Secondary Antibody, Novus Biologicals™
SDP

Pig Polyclonal Antibody

Antigen IgE Fc
Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purification Method Ion exchange chromatography
Conjugate Unconjugated
Target Species Human
Host Species Pig
Applications Dot Blot,ELISA,Immunoblot,Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Paraffin),Western Blot
Form Lyophilized
Formulation Lyophilized from PBS (pH 7.2) with No Preservative
Classification Polyclonal
Immunogen Purified homogenous IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Primary or Secondary Secondary
4,844

Pig anti-Guinea Pig IgG (H+L) Secondary Antibody, Novus Biologicals™
SDP

Pig Polyclonal Antibody

Antigen IgG (H+L)
Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purification Method Ion exchange chromatography
Conjugate Unconjugated
Target Species Guinea Pig
Host Species Pig
Applications Dot Blot,ELISA,Immunoblot,Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Paraffin),Western Blot
Form Lyophilized
Formulation Lyophilized from PBS (pH 7.2) with No Preservative
Classification Polyclonal
Immunogen Purified normal IgG isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Primary or Secondary Secondary
4,845

Donkey anti-Human IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Donkey
Conjugate Janelia Fluor 669
Applications Western Blot,ELISA,Immunohistochemistry
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Human IgG whole molecule
Primary or Secondary Secondary